Forest bats of eastern North America select habitats for roosting, foraging, and winter hibernation/migration over a myriad of scales. An understanding of forest-bat habitat use over scales of time and space is important for their conservation and management. The papers in this Special Section report studies of bat habitat use across multiple scales from locations across the eastern forests of North America. The consensus of the studies in the Special Section is that the larger portion of the variability in bat habitat use occurs at the smaller scales of sites (roost trees) and stands (foraging areas). Nevertheless, it was also recognized that these features occur discontinuously across larger-scale watersheds and landscapes.